subcellular fractionation

David J. Eide deide at D.UMN.EDU
Fri Aug 25 08:39:31 EST 1995


Hi Jonathon,


We've had success with doing subcellular fractionation by sucrose gradients.  A 
good reference is Kolling and Hollenberg, EMBO 13:  3261 (1994).  I'd also be 
happy to lend any advice that I can.

Dave Eide  

In message <41japj$oou at news.service.uci.edu> Jonathan Claypool writes:
> Hello, What do people think are the best protocols for yeast subcellular 
> fractionation?  I am 
> looking to determine the location of components of a yeast retrotransposon.  
> Any help is 
> appreciated.
> 
>     
> 
> Sincerely,
>            Jonathan Claypool
>            eabg153 at rigel.oac.uci.edu








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