Homologous recombination

Trueheart 71562.2253 at COMPUSERVE.COM
Tue Aug 29 10:41:36 EST 1995


Dan,
I have also done this with success with a 2mu backbone and no purification
(simple laziness).  It worked fine, though in my case I was targetting a
functional TRP1 gene and was able to screen the transformants for Trp- (I guess
you can't do this as your strain is already Trp-).   More than half were
correct.

Josh Trueheart 
Cadus




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