selecting for growth arrest

Tomas Drgon tomasd at bdg10.niddk.nih.gov
Wed Aug 30 17:20:57 EST 1995


In article <sbv-300895102145 at path22304.path.cwru.edu>, sbv at pop.cwru.edu
(Scott Vande Pol) wrote:

> We want to select in yeast for temperature sensitive mutants in our gene
> that should result in the yeast failing to grow at the restrictive
> temperature.  Is there a good drug (ie a nucleotide analog) to kill those
> yeast that continue to grow at the restrictive temperature in order to
> enrich for the population that failed to grow at the restrictive
> temperature? One could then wash out the drug and recover those yeasts at
> the permissive temperature.  ts mutants in this gene seem to be rare and we
> would like to enrich for them.  Any experience on this would be
> appreciated!
> 
> -- 
> Scott Vande Pol
> Case Western Reserve University
> Department of Pathology
> Cleveland Oh. 44106
> sbv at pop.cwru.edu

Hi Scott.
I will post a summary on the topic. It was discussed here a couple of weeks ago.

Tomas

-- 
I speak for nobody but myself and my pet frog.



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