titrating gal1 promoter

Bruce G Adams bruce at hawaii.edu
Thu Aug 31 18:52:24 EST 1995


Dear yeasties--a note on titrating the gal1 promoter by playing with gluocse
and galactose concentrations: once you have induced the gal1 gene (kinase)
with galactose and the galactose is still present, the addition of gluocse
will repress the expression of Gal1, but not shut it down. See Adams, B.G.
1972. J. bacteriol. 111:308-315, Figure 6. Presumably, once the regulon is
indcued, it will stay that way in the presence of galactose due to the
induction of the permease system, involving the GAl2 gene. Aloha



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