methylene blue

R.J. Davenport rjd23 at CUS.CAM.AC.UK
Thu Dec 7 05:54:56 EST 1995


dear yeast people, 
i need a screen for yeast mortality on agar plates, which is non-toxic 
and will distinguish cells in growth arrest from truly dead ones. I have 
been trying to use a methylene blue method (Iida et al., 1994), but all my 
cells turn blue whether i've threatened their lives or not.
So, does anyone know what exactly methylene blue is staining in dead 
yeast, and whether it is toxic over several days?
And, any other bright ideas for easily detecting survival on agar plates 
(in the absence of growth)?
thanks very much!

Romola Davenport



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