3 AT selection for 2-hybrid

S.L. Rennie rennie at bldghsc.lan1.umanitoba.ca
Mon Dec 11 13:37:55 EST 1995


In article <4a4fo7$gee at delphi.bc.edu>, Sarah Waywell
<waywell at hermes.bc.edu> wrote:

> I am using 3 amino-triazole as a selection for protein-protein
> interaction. Right now I am testing 25mM and 50mM concentrations and have
> been making my plates by adding the chemical straight to the media before
> autoclaving.  Is this the correct way to use it or am I supposed to make
> a stock solution and either autoclave separately or filter sterilize and
> add to the media after autoclaving.  Info would be greatly appreciated.
> Thanks,
> Sarah Waywell
> 
> e-mail
> waywell at hermes.bc.edu


Hi Sarah
   We always make a 2.5 M stock solution and add to the media after 
autoclaving.  Warning this stuff can take forever!! to filter sterilize.
Have no ref. for you on this though...Sorry.

Regards
Sharon

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