yeast genomic DNA

S.L. Rennie rennie at bldghsc.lan1.umanitoba.ca
Mon Dec 11 13:40:26 EST 1995


In article <Pine.SUN.3.91.951211142429.5985A-100000 at nwg.nectec.or.th>,
poonso at NWG.NECTEC.OR.TH (Poonsook Orprayoon) wrote:

> Hello everybody,
> 
> I wonder if anyone could tell me the simple method for extracting yeast 
> chromosomal DNA or total DNA.  We have tried using the glassbead to break 
> cell but the DNA we got seem to be too small. I think that the DNA may be 
> chopped or this method works only with the plasmid DNA.  Anyone knows, 
> plase comment.
> 
> Poonsook
> E-mail:poonso at nwg.nectec.or.th

We always use the glassbead method and have consistently used this DNA
for analysis.  The amount you get isn't much but it shouldn't be sheared
or anything??

Regards
Sharon

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