yeast genomic DNA

S.L. Rennie rennie at
Mon Dec 11 13:40:26 EST 1995

In article <Pine.SUN.3.91.951211142429.5985A-100000 at>,
poonso at NWG.NECTEC.OR.TH (Poonsook Orprayoon) wrote:

> Hello everybody,
> I wonder if anyone could tell me the simple method for extracting yeast 
> chromosomal DNA or total DNA.  We have tried using the glassbead to break 
> cell but the DNA we got seem to be too small. I think that the DNA may be 
> chopped or this method works only with the plasmid DNA.  Anyone knows, 
> plase comment.
> Poonsook
> E-mail:poonso at

We always use the glassbead method and have consistently used this DNA
for analysis.  The amount you get isn't much but it shouldn't be sheared
or anything??



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