spheroplast regeneration

jos urbanus urbanus at chem.vu.nl
Mon Dec 18 06:32:59 EST 1995


Hi, everybody
I am from the biochemical department of the VU, Amsterdam, and trying to 
develop different kinds of toxicity assays with yeast. In order to 
increase the availability of a drug to a cell, spheroplasts can play an 
important role in combination with certain kind of drugs. In such an 
assay it would be essential that most cells stay alive and keep on 
proliferating.
To produce spheroplasts from normal cells is very easy, but until now I 
have not been able to regenerate these cells. In an 0.7 M sorbitol 
YNB-medium spheroplast do not generate a cell wall, produce enormous 
vacuoles and finally blow themselves up, I guess.
So, if anybody has experience with spheroplast regeneration, please give  
me advice or a protocol.





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