lac promoter activity in yeast?

Yogesh K Sharma ysharma at magnus.acs.ohio-state.edu
Mon Dec 18 20:11:54 EST 1995


Dear yeast netters:
I am trying to complement a yeast mutant with arabidopsis cDNA clones.  The 
arabidopsis cDNA library is constructed in lambda YES shuttle vector.  YES has 
lac and gal promoters that drive expression in E. Coli and yeast respectively.  
Lac and gal promoters are flanking the cloned cDNA inserts.on the opposite 
ends.Here comes the puzzling part: one of the arabidopsis clones that 
complemented the yeast mutant has the cDNA insert  in the orientation that it 
is being driven by the lac promoter ie beginning of the cDNA is next to lac 
promoter.  How is this cDNA able to complement the yeast mutant. Does lac 
promoter has any activity in yeast? or alternativley antisense mRNA is being 
produced due to gal promoter activity. What is the most plausible explanation ? 
Any replies and suggestions will be highly appreciated.

Yogesh Sharma



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