Storage of Sporulated culture

Michael Lichten lichten at
Tue Dec 19 07:47:37 EST 1995

In article <Pine.A32.3.91.951218175611.65985B-100000 at>,
fgdnobre at (Francisco G da Nobrega ib - bio 7588) wrote:

> Dear yeast people:
> I would like to have advice on the best way to keep for  long time =20
> sporulated S. cerevisiae diploids suitable for sampling, glusulase=20
> treatment and spore analysis.
> Does it keep well in liquid sporulation medium?
> Is it possible to freeze away at -80oC (plus glycerol) without rupture of=
> =20
> the ascus wall?

I have kept ascii in liquid sporulation medium for a couple of weeks at
4oC with no apparent ill effects.

Michael Lichten
lichten at

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