colony hybridization

Michael Lichten lichten at HELIX.NIH.GOV
Wed Feb 8 09:47:50 EST 1995


Kevin Piers writes,

>Dear Netters,
>
>Does anyone out there know of a reliable method for preparing yeast
>colonies on NC filters for the purpose of probing them with a specific
>piece of DNA?  Any information is greatly appreciated and can be sent
>directly to me.
>
I have found that the protocol given in the Cold Spring Harbor Methods in
Yeast Genetics manual (Rose, Winston, Hieter are the authors of my version)
works well.  I generally replica plate onto filters on YPD plates, let grow
4-6 hr at 30C, and then lyse as they describe.

Michael Lichten
lichten at helix.nih.gov






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