phloxin B: follow-up
belhumep at ERE.UMontreal.CA
Thu Feb 9 12:47:15 EST 1995
Here's the latest (and only!) answer I received: that speaks for itself.
I wish to thank Mike Moser for explaining how phloxin B works. I must say
that I tried the pombe concentration (2.5 mg per L, sterilizing the 0.5%
stock solution by filtration) and it seems to work too: as you'll see
below, Vickie uses this stain at 20 mg per L but it is autoclaved with
the medium. Any concentration will do!!
Again, thanks everybody
Microbiology, U. of Montreal
belhumep at ere.umontreal.ca
Date: Thu, 9 Feb 1995 10:30:33 -0700
From: Vickie Peck <vpeck at triton.unm.edu>
To: belhumep at ERE.UMontreal.CA
Subject: Phloxine B Screening
Dear Dr. Belhumeur;
I am responding to your request for information regarding phloxine
B . We have used phloxine B to screen for stationary phase mutations in S.
cerevisiae. Phloxine B is added directly to the medium at 20 mg/l, which
is autoclaved as usual. For more information, see Cannon et al., Genetics
113:247-264 (1986). Good luck!
Dept. of Biology
] University of New Mexico
Albuquerque, NM 87131
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