Question from yeast novice

Michael Lichten lichten at HELIX.NIH.GOV
Sun Feb 12 17:35:12 EST 1995

On Sat, 11 Feb 1995 APARKER at MAINE.MAINE.EDU wrote:

> Howdy there!
> I'm interested in long-term storage of some cultures of Saccharomyces sp.
> (guess why?).  I am familiar from my work with bacteria with glycerol
> storage buffers that allow viable storage for up to several years after
> snap-freezing at -80 C; I'm assuming (hoping) there's a comparable
> protocol for yeasts, but don't know where to find it.
> Any pointers are greatly appreciated in advance!
Just make 'em up to 25% glycerol and put them in the -80 C freezer.  We 
use 50% glycerol, but only because that's what we use for E. coli, and 
it's too much trouble to make up two different solutions....

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