3-AT, YMM? YPD?
mbrown at fred
Tue Feb 14 20:39:13 EST 1995
I don't know if it's best, but I use synthetic media, e.g. complete
synthetic minus hisitidine etc. I autoclave the media first, then let it
cool some before adding the appropriate amound of 3AT. I make up the 3AT
in H2O as a 1 M stock and store it at -20. I have found that adding 35
glucose to the media instead of my usual 2% increases plating efficiency
on this media, but this is somewhat anecdotal. It is crucial to leave the
histidine out of the media; thus, I would not expect rich media such as
YEPD to work.
mbrown at fred.fhcrc.org
ROSENKRANTZ at Gems.VCU.EDU wrote:
: Can someone tell me the best plate to use 3 aminotriazole on and
: directions or a citation? Thanks
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