fragments of LEU2 that complement

Charles Brenner brenner at rose.brandeis.edu
Thu Feb 16 11:01:05 EST 1995


Does anyone know if the 2 kb Hpa I-Sal I fragment of LEU2 complements
as a single copy integrant?  I know that fragment(s) shorter than the
2.2 kb Xho I-Sal I fragment are used in leu2d vectors to select for
high copy number.  What fragments are those and will Hpa-Sal work in a
knockout?

Thanks,
Charlie

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