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plasmids with 2 2 micron circles

George Theodoris ez003450 at bullwinkle.ucdavis.edu
Wed Feb 15 20:23:36 EST 1995

Are plasmids with more than 1 2micron circles  stable in yeast? I was 
subcloning into pRS424 and some of my miniscreens, in addition to having 
my insert, also have concatamers of pRS424. I transformed them into 
yeast and the gene seems to be expressed but I'm worried about the yeast 
rearranging the plasmids to get rid of the extra 2micron circles.  

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