About competent cells.

[Jeff Simon]

There is a company that sells a Frozen-EZ Yeast Transformation kit.

Zymos Research
625 W. Katella Ave., Suite 32
Orange, CA 92667
Phone	(714) 288-9682
Fax		(714) 288-9643
E-mail	zymo at kaiwan.com

We have used this kit and have had good results.  Use only 1/5 of the volumes they recommend and plate the whole transformation.  One microliter of an E. coli miniprep can give one hundred colonies.

Jeff Simon  Oregon Health Sciences University, Portland, OR
simonj at ohsu.edu