About competent cells.

mpk6 MPK6 at psuvm.psu.edu
Tue Feb 21 11:18:30 EST 1995


A DMSO-enhanced LiOAc transformation protocol was described in NAR
(1991).  19,5791.  They reported that they could use competent cells
after they were frozen in 5% DMSO.  I find that it works quite well in
my hands, however, as they indicated, the efficiency is only in the 10e4
range.

Mike Kladde
PennState Univ.
308 Althouse Lab.
University Park, PA 16802



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