Are Terminators Necessary

Maor Maor
Thu Feb 23 12:37:57 EST 1995


Hello Netters.  I am new in Yeast work. I'd like to introduce and express
a plant cDNA (with stop codon, but without any further 3'seq.) in yeast.
My questions are :1- I've heard that terminators are not required, is that true
?                 2- Can someone send me a LEU marker  plasmid having a 
constitutive or inducible promoter, multicloning sites, and a terminator.

Thank you, Maor Bar-Peled, Plant Research Lab. Mi. 



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