Need meth. to distinguish exo/endo glucanase

[Francis Ouellette]

Christian Archambault (popa0215 at PO-Box.McGill.CA) wrote:

> I need a method to characherize the protein we purified 
> as an endo or exo beta-1,3 glucanase.  I would appreciate any 
> method or comments.

Dear Christian

check on the kinetics of laminarin degradation vs pachyman 
hydrolysis. Go see Gordon MacLaclan in the bilogy department
at McGill.  I have worked on 1-3,beta-glucanases in that lab as an
undergrad, and somebody had purified these two enzymes (endo
vs exo) from pea.

Also note that there is a bionet GLYCOSCI newsgroup
(bionet.glycosci).

regards,

francis

--
| B.F. Francis Ouellette  
|
| francis at ncbi.nlm.nih.gov