Need meth. to distinguish exo/endo glucanase
francis at borduas.nlm.nih.gov
Tue Feb 28 10:02:36 EST 1995
Christian Archambault (popa0215 at PO-Box.McGill.CA) wrote:
> I need a method to characherize the protein we purified
> as an endo or exo beta-1,3 glucanase. I would appreciate any
> method or comments.
check on the kinetics of laminarin degradation vs pachyman
hydrolysis. Go see Gordon MacLaclan in the bilogy department
at McGill. I have worked on 1-3,beta-glucanases in that lab as an
undergrad, and somebody had purified these two enzymes (endo
vs exo) from pea.
Also note that there is a bionet GLYCOSCI newsgroup
| B.F. Francis Ouellette
| francis at ncbi.nlm.nih.gov
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