ade2 and red colour

Wilfried Rosoll imsd007 at wrzx12.rz.uni-wuerzburg.de
Fri Feb 24 12:23:22 EST 1995


kohlwein at fscm1.dnet.tu-graz.ac.at wrote:
: Is there a way to increase the intensity of red/pink colour development
: of an ade1 or ade2 mutant on YPD plates or selective media ?
: Our w303-1A derivative (ade2 rho+/Pet+) is only very light pink.

 Try adding more glucose to your YPD (4 or 6%) or use prespore-plates
(10% glucose!). This helps for our W303 derived strains but I have not
tested this with selective media. Storing the plates at +4 also improves
colour development.  

 Wilfried ROSSOLL
 imsd007 at rzbox.uni-wuerzburg.de



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