3-Amino-Triazole Concentration

Michael Moser moser at U.WASHINGTON.EDU
Tue Jan 3 13:17:02 EST 1995


Dear Beasties,
	I am attempting a 2 hybrid screen for proteins that interact with 
a very acidic protein.  Unfortunately my LexA fusion seems to be a decent 
transcriptional activator on its own.  I would like to try 3-AT to reduce 
my background of His+ colonies.
	I would appreciate your input on how to make up a stock solution 
of 3-AT.  What is the best solvent to use (H2O?), and what is the maximum 
concentration of 3-AT I can get into solution.  Thanks in advance for 
your help!
Sincerely,

Mike Moser                                             Tel: 206-543-5354
Department of Biochemistry  SJ-70                      FAX: 206-685-1792
University of Washington                          moser at u.washington.edu
Seattle, WA  98195                          Make peace my beast is yeast




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