Removal of Non-sporulated Diploids

Geoff Kornfeld geoffk at AIX00.CSD.UNSW.OZ.AU
Wed Jan 4 16:53:31 EST 1995


The original reference is Dawes & Hardie, Mol. Gen. Genet. 131, 281-289 (1974).
The ether resistance of the diploids and the ether sensitivity of the spores
varies between strains so it is best to do some trials to determine the best
time & temperature to use.  We use the method a lot without any problems.


Geoff Kornfeld




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