3-AT Concentration Summary

[Michael Moser]

Dear Yeasties
	I had a request to summarize so here are the best 2 answers:

John R. Geiser wrote:

>I've been making up a 2.5 M solution of 3 AT in dH20.  Seems close to the
>saturation point but I'm not sure what saturation is.  I know that 5 M
>will not work.

Mark Johnston wrote:

>I simply add the necessary amount of solid AT directly to the autoclaved
>media before pouring the plates.  I've never had a problem.

Hope this info is of some use.
Sincerely,

Mike Moser                                             Tel: 206-543-5354
Department of Biochemistry  SJ-70                      FAX: 206-685-1792
University of Washington                          moser at u.washington.edu
Seattle, WA  98195                          Make peace my beast is yeast