yeast colony PCR

pulitzer at PLAUTO.CSATA.IT pulitzer at PLAUTO.CSATA.IT
Tue Jan 17 15:26:45 EST 1995


>Any good protocols? citations?  THanks
Method described in Sathe et al NucL Ac. Res 19:4775 ( sorry I don't have
the year) works fine. We make a patch, pick two large loop-fulls wash with
1 ml H20 and proceed as per protocol. Cheers.

P.S. We resuspend after washing in c. 20 ul and use 1-2 ul per 100 ul
reaction.

John F. Pulitzer
Dept. of Genetics and Molecular Biology
U. of Naples "Federico II"
via Mezzocannone, 8
80134 Naples (Italy)
(tel. 3981-552-6208; ext. 321)
(FAX 3981-552-7950)











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