5-FOA

Michael Lichten lichten at HELIX.NIH.GOV
Thu Jan 26 09:29:12 EST 1995


>Hi everyone
>I need to do some plasmid shuffle/gene replacement experiments, using the
>5-FOA negative selection for loss of the URA3 marker.  This stuff (FOA) is
>horrifically expensive!  I have heard a rumour that there is a recipe for
>5-FOA plates which enables one to use a lot less of the compound and get
>the same selection - does anyone out there have a ref for this or any info
>on the technique? post it or mail to me, and I will post a compilation of
>the answers for everyone.
>Thanks,
> colin macdiarmid
>
>macdiarm at sbsu1.auckland.ac.nz

UI  - 92116644
AU  - McCusker JH ; Davis RW
TI  - The use of proline as a nitrogen source causes hypersensitivity
      to, and allows more economical use of 5FOA in Saccharomyces
      cerevisiae.
SO  - Yeast 1991 Aug-Sep;7(6):607-8


Michael Lichten
lichten at helix.nih.gov






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