Galactose Purity for GAL10-CYC1 Induction?

Anthony Tomlinson tomlinson at pplros.demon.co.uk
Thu Jan 26 10:00:26 EST 1995


In article <sjszarka-2001951516100001 at canola.bio.ucalgary.ca> Steven
Szarka, sjszarka at acs.ucalgary.ca writes:
>For galactose induction of a GAL10-CYC1 hybrid promoter,
>how pure does the galactose need to be? Is 1-2% glucose 
>in the galactose stock an acceptable level?

The GAL10 promoter is repressed by glucose, but the amounts
you mention are not significant.  It should work fine.

Anthony



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