Galactose Purity for GAL10-CYC1 Induction?

Anthony Tomlinson tomlinson at
Thu Jan 26 10:00:26 EST 1995

In article <sjszarka-2001951516100001 at> Steven
Szarka, sjszarka at writes:
>For galactose induction of a GAL10-CYC1 hybrid promoter,
>how pure does the galactose need to be? Is 1-2% glucose 
>in the galactose stock an acceptable level?

The GAL10 promoter is repressed by glucose, but the amounts
you mention are not significant.  It should work fine.


More information about the Yeast mailing list