gel shifts with S. cerevisiae

Wolfgang Hammerschmidt hammers at
Thu Jan 26 10:39:13 EST 1995

Hi folks,

does anybody have specific information on making extracts from S.c. for
gel shifts? Are glass beads and a generic buffer containing a variety of
proteinase inhibitors o.k.? Any experience with freeze/thaw cycles? What
would be the expected protein content? 

We are trying to set up a one-hybrid system for specific DNA-binding
transcription factors and we would like to be able to screen small liquid
cultures (about 5 ml) for shift activities.

Thanks much.

Wolfgang Hammerschmidt
GSF-Institut fuer Klinische Molekularbiologie
Marchioninistr. 25
D-81377 Muenchen

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