Selective killing of dividing yeast

AL R. PLUMMER aplummer at sprint.uccs.edu
Wed Jul 5 15:25:49 EST 1995


TOMASKA at fns.uniba.sk wrote:
: Can somebody suggest a method of selective killing of groowing as
: opossed too non-dividing yeast cells?

: Lubo Tomaska
: Dept. Genetics
: Faculty of Natural Sci.
: Comenius University
: Bratislava, Slovakia

I have used the method of Snow as described in:
 
Snow,R. Nature 211:206-207 (1966)
An enrichment method for auxotrophic yeast mutants using the antibiotic
nystatin.

I have had success isolating many mutants based on nystatins ability
to selectively kill non-growing cells.

		-Al(aplummer at sprint.uccs.edu)



More information about the Yeast mailing list