elutriators for S. cerevisiae

[Dan Gottschling]

Hi!

	Our lab is in the market for an elutriation device to isolate yeast 
cells for cell cycle studies related to silencing.  We currently have a 
Beckman high speed centrifuge and were planning on purchasing a Beckman 
JE-6B Elutriator rotor plus accessories.  In addition we need to by a 
pump and suitable diameter tubing.  Has anyone out there had experience 
with this equipment for separating yeast cells?  We'd appreciate 
learning about what pumps and tubing give good results, as well as 
running conditions to get good separation.  If you have experience with 
other elutriation devices I'd appreciate hearing about the options that 
work well.  

	Thanks for your help.

Dan Gottschling
MGCB - University of Chicago
deg5 at midway.uchicago.edu
FAX:(312) 702-3172