Plasmid stability

Ian A. York york at mbcrr.dfci.harvard.edu
Wed Jul 5 15:11:15 EST 1995


This may be too broad a question, but what the heck.  How stably are 
plasmids maintained in S cervisiae?  That is, if you stop selective 
pressure, and assuming that the plasmid isn't expressing something toxic, 
what kind of half-life will it show?  Will most of the plasmid be lost 
after 6 hours in culture?  24 hours?  A week?  Or is it unpredicatable, 
or does it depend on other conditions?  I'm specifically thinking of 2 
micron plasmids, but any information would be helpful.

Ian
-- 
Ian York   (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921     Fax  (617)-632-2627




More information about the Yeast mailing list