gap repair

Jaap Venema venema at EMBL-Heidelberg.DE
Fri Jul 7 09:42:09 EST 1995

Dear Buddies,

I would like to clone chromosomal mutant alleles using a gapped plasmid.
there are two aspects I am not entirely syre about:

(1) does it matter when the two ends of the linear plasmid are compatible,
i.e. is ligation vs. recombination a serious problem?

(2) does the type of vector (CEn vs. 2um) matter?

Any info can be emailed to the network or directly to me
(venema at Thank you very much!

Jaap Venema

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