gap repair

Jaap Venema venema at EMBL-Heidelberg.DE
Fri Jul 7 09:42:09 EST 1995


Dear Buddies,

I would like to clone chromosomal mutant alleles using a gapped plasmid.
there are two aspects I am not entirely syre about:

(1) does it matter when the two ends of the linear plasmid are compatible,
i.e. is ligation vs. recombination a serious problem?

(2) does the type of vector (CEn vs. 2um) matter?

Any info can be emailed to the network or directly to me
(venema at embl-heidelberg.de). Thank you very much!

Jaap Venema





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