Vector for Library Co
tod.lorenz at ghawk.com
Sat Jul 8 21:45:00 EST 1995
-=> Quoting Al R. Plummer to All <=-
ARP> ÿ#FROM :aplummer at sprint.uccs.edu
ARP> Organization: University of Colorado at Boulder
ARP> I am considering constructing a S. cerevisiae genomic libray and would
ARP> like opinions on the vector to work with. I know that the Rose/
ARP> Botstein and Carlson/Botstein use YCp50 and Yep24 vectors
ARP> respectively. I would like to know if the Hieter Series of multicopy
ARP> vectors (pRS 423, 424, 425 and 426) are a viable option for library
ARP> construction. I guess I should mention that I want a multicopy library.
ARP> I would also like to hear about any protocols for library construction
ARP> other than those found in the Guide to Yeast Genetics and Molecular
ARP> Biology, along with any tips and suggestions.
I have used a YCp50 library for mutant complementation
with no problems at all, I think it was cut with bamI...
works good, tastes great. Seems like we
got it somewhere commercially, Andy Keesler I believe would
know, but it is easy enough to make yourself a library using
either plasmid.....If you plan to do sequencing why not make a
library in pBlueScript, hmmmm, would that
work, yep I think so.....
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