Q re. pJG4-5
EA_Golemis at fccc.edu
Mon Jul 10 08:49:36 EST 1995
Both of the forward primers suggested for sequencing from the forward
direction work very well. That listed in the Red Book is a bit more
5', in case you happen to have trouble reading sequence close to the
primer. So, no particular preference.
The downstream primer is a different matter. In my hands that primer
does not work well. I've just done a bit of checking, and I suspect
that there may be an additional point error beside the one you pointed
out (thank you!). I will attempt to find a corrected sequence over the
next day or so, and I will re-post it here. Please email me if you have
any further questions!
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