DAPI staining

Hoffmacs Hoffmacs at HERMES.BC.EDU
Tue Jul 11 14:48:32 EST 1995


Hi fellow netters,
I want to photograph some S. pombe cells, both phase and DAPI-stained.  Is
there a fast and simple method for fixing and permeablizing cells for
DAPI-staining relative to the procedures used for immunofluorescense?  Thanks
in advance.
Charlie Hoffman
"I'd rather be fission."
P.S.  When trashing the "Hoffman and Winston" protocol for plasmid rescue
from yeast, please refer to it as the "Smash and Grab" technique.  Thank you.



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