BrdUrd

[M. K. Raghuraman]

In article <3uoqvn$q5v at fox.ksu.ksu.edu>, bethmont at ksu.ksu.edu (Beth A
Montelone) wrote:

> Saccharomyces cerevisiae do not incorporate BrdUrd since they lack
> thymidine kinase and can't carry out the salvage pathway which allow
> mammalian cells to use this analog.  Don't know about pombe.  Anyone?
> 


...but S. cerevisiae cells that have a foreign tk gene (such as the HSV
TK) will incorporate BrdU.  Here's a reference:

AU   Sclafani-R-A.  Fangman-W-L.
TI   Thymidine utilization by tut mutants and facile cloning of
     mutant alleles by plasmid conversion in S. cerevisiae.
SO   Genetics.  1986 Nov.  114(3).  P 753-67.
JT   GENETICS.

Cheers,
Raghu <raghu at u.washington.edu>