M. K. Raghuraman
raghu at u.washington.edu
Sat Jul 22 04:55:54 EST 1995
In article <3uoqvn$q5v at fox.ksu.ksu.edu>, bethmont at ksu.ksu.edu (Beth A
> Saccharomyces cerevisiae do not incorporate BrdUrd since they lack
> thymidine kinase and can't carry out the salvage pathway which allow
> mammalian cells to use this analog. Don't know about pombe. Anyone?
...but S. cerevisiae cells that have a foreign tk gene (such as the HSV
TK) will incorporate BrdU. Here's a reference:
AU Sclafani-R-A. Fangman-W-L.
TI Thymidine utilization by tut mutants and facile cloning of
mutant alleles by plasmid conversion in S. cerevisiae.
SO Genetics. 1986 Nov. 114(3). P 753-67.
Raghu <raghu at u.washington.edu>
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