M. K. Raghuraman raghu at
Sat Jul 22 04:55:54 EST 1995

In article <3uoqvn$q5v at>, bethmont at (Beth A
Montelone) wrote:

> Saccharomyces cerevisiae do not incorporate BrdUrd since they lack
> thymidine kinase and can't carry out the salvage pathway which allow
> mammalian cells to use this analog.  Don't know about pombe.  Anyone?

...but S. cerevisiae cells that have a foreign tk gene (such as the HSV
TK) will incorporate BrdU.  Here's a reference:

AU   Sclafani-R-A.  Fangman-W-L.
TI   Thymidine utilization by tut mutants and facile cloning of
     mutant alleles by plasmid conversion in S. cerevisiae.
SO   Genetics.  1986 Nov.  114(3).  P 753-67.

Raghu <raghu at>

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