Yeast two hybrid

Bert Popping Bert.Popping at durham.ac.uk
Mon Jul 24 08:25:04 EST 1995


cgdn at uhura.cc.rochester.edu (Craig Duncan) wrote:
>Dear Ms. Timblin:
>     I am a little confused by you question. The nature of the two
>hybrid system implies that the activation domain is present and being
>expressed in every cell. Positive signals come from interactions
>between the activation domain and the DNA binding domain mediated
>by the other portions of the hybrid proteins. Thus PCR of the
>activation domain gene or mRNA will not tell you anything useful.

..unless one screens a library and uses the primer for direct 
sequencing of the PCR product

Bert Popping, UK





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