sandy at icr.pt.cyanamid.COM
Mon Jul 24 16:21:44 EST 1995
>Here is another question now, since I apparently picked up a suppressor
>somewhere (these guys do sector red on YEPD):
>Why are these cells quite red on minus Leu but mostly white on minus His,
>Ura, or Lys?
My quick'n'dirty answer is different amounts of adenine in your different
media mixes. The red color development is very senstive to this. (More
adenine=later or little color).
Looking more deeply, I wonder if there is something else going on? Are
there other ochre-suppressible markers in your strain? Is the suppressor
on a plasmid (as sectoring red on YEPD suggests?). Tell me more!
bethmont at ksu.ksu.edu
Only lys2-801 (?) and they are really lys minus. No plasmids (that I know
Sanford Silverman >Opinions expressed here are my own<
silvermans at pt.cyanamid.com "Yeast is Best"
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