PCR from yeast plasmid

Nicholas Kaplinsky kaplinsk at ohsu.edu
Tue Jul 25 20:07:02 EST 1995


Hi,
I am trying to do PCR from yeast plasmids and am not having any success.  I have made 'yeast extract' according to the protocol in the Red Book (current protocols in molecular biology), I can transform E. Coli with the extract, but for some reason my PCR is not working.  Is there something that I am missing?  Any help would be greatly appreciated, Thank You
Nick Kaplinsky  



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