ADH promoter control in S.cerevisiae?
souza at alf.biochem.mpg.de
Mon Jun 5 05:56:16 EST 1995
Steven Joseph Szarka (sjszarka at acs.ucalgary.ca) wrote:
: Is the ADH promoter considered constitutive?, or is it up regulated
: during anoxia?
: I seem to get good expression in 5 ml tube cultures but lose expression
: when I scale up to 100 mls in 250 mls flasks.
: Thanks for any help
Sorry about the previous post!
If anoxia were a condition for expression from the ADH
promoter, a lot of interactor hunt people would be in trouble!
The Red Book (article by Golemis, Gyuris & Brent) describes the
alcohol dehydrogenase promoter as "strong constitutive".
Hope this helps though I still can't understand why you don't get
expression in the large cultures.
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