SUP4-0 transformation

ANDREI POPOV ANDREI.POPOV at bbsrc.ac.uk
Tue Jun 6 21:33:19 EST 1995


Hi
you can cure your strain from psi+  by growing
them in the presence of guanidine hydrochloride.

Look at Yeast 4,159 (1988)

BTW, if I do not mind D.Burke did not  state
that you cannot transform psi+ strains with a plasmid
which carries SUP4-O, the point was that you should not
use such a plasmid to estimate the efficiency of
your transformation procedure because it would be much lower
than that using YEp24 for example.
Regards
Andrei




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