ADH1 promoter

Joanne Tornow jtornow at whale.st.usm.edu
Tue Jun 6 16:49:35 EST 1995


It's been known for quite a while that ADH1 transcription is NOT constitutive 
but regulated according to carbon source. This was first reported by Denis et 
al. (JBC 258:1165-1171 [1983]), and was confirmed by us in an analysis of the 
contribution of RAP1 and GCR1 to ADH1 promoter function (RAP1/GCR1 
interdependent activation accounts for most of ADH1 mRNA production in cells 
grown either on a fermentable or a nonfermentable carbon source; see Gene 
90:79-85 [1990] and MCB 10:859-862 [1990]). We have fused the ADH1 promoter to 
a heterologous reporter gene, which results in approximately 5-fold higher 
activation in glucose-grown cells than in pyruvate- or ethanol-grown cells, 
indicating that it is the ADH1 promoter per se that confers carbon source 
regulation (unpublished data). Our unpublished results also indicate that ADH1 
transcription initiation is regulated according to growth phase, such that the 
highest levels occur in early log and a five-fold down effect is seen by 
stationary phase. Steve Kron has recently suggested that it makes sense for 
ADH1 levels to be high in the presence of accumulated ethanol. He must be 
referring to ADH enzyme activity, not expression of the ADH1 gene, since it is 
a different ADH-encoding gene, ADH2, that is transcriptionally derepressed and 
responsible for the extremely high levels of ADH in cells grown on ethanol or 
after diauxic shift in rich media. ADH2 expression is negligible in 
glucose-grown cells.


George Santangelo
Joanne Tornow
University of Southern Mississippi
santnglo at whale.st.usm.edu
jtornow at whale.st.usm.edu



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