[HELP] Electroporation Protocol

Guy Oshiro oshiro_G at defiance.hsc.colorado.edu
Wed Jun 14 13:34:15 EST 1995

You need an electroporator that is capable of attaining 1500 volts.  I
need to double check what the exact field strength is.  We use .2 cm
gap cuvettes and we get great results -- about 1-5 X 10^4-5 cfu/ug.

Also check the yeast bible by Gutherie and Fink for a more detailed

Guy Oshiro
EMAIL: Oshiro_G at defiance.hsc.colorado.edu

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