[HELP] Electroporation Protocol

Guy Oshiro oshiro_G at defiance.hsc.colorado.edu
Wed Jun 14 13:34:15 EST 1995


You need an electroporator that is capable of attaining 1500 volts.  I
need to double check what the exact field strength is.  We use .2 cm
gap cuvettes and we get great results -- about 1-5 X 10^4-5 cfu/ug.

Also check the yeast bible by Gutherie and Fink for a more detailed
protocol.

Guy Oshiro
EMAIL: Oshiro_G at defiance.hsc.colorado.edu

  /\  /\    /\
 /  \/  \/\/  \ Science in the Rockies.
/    \   \/    \



More information about the Yeast mailing list