Gal vector for amino-His tagging
brenner at rose.brandeis.edu
Wed Jun 14 17:36:37 EST 1995
Is there a Gal vector that is set up for amino-terminal
His-tagged proteins? The ideal vector would encode Met and ~6
Histidines follwed by a unique Nde I site. Nde I encodes HisMet so
one would provide one's coding sequences with Nde I at the initiator
Met. This seems too good not to have been done already!
Thanks in advance.
Rosenstiel Center, rm 610 phone (617) 736-4944
Brandeis University fax (617) 736-2405
Waltham, MA 02254-9110 brenner at auriga.rose.brandeis.edu
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