Gal vector for amino-His tagging

Charles Brenner brenner at rose.brandeis.edu
Wed Jun 14 17:36:37 EST 1995


Dear Buddies,
	Is there a Gal vector that is set up for amino-terminal
His-tagged proteins?  The ideal vector would encode Met and ~6
Histidines follwed by a unique Nde I site.  Nde I encodes HisMet so
one would provide one's coding sequences with Nde I at the initiator
Met.  This seems too good not to have been done already!

	Thanks in advance.
Charlie Brenner

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