Gal vector for amino-His tagging

Charles Brenner brenner at
Wed Jun 14 17:36:37 EST 1995

Dear Buddies,
	Is there a Gal vector that is set up for amino-terminal
His-tagged proteins?  The ideal vector would encode Met and ~6
Histidines follwed by a unique Nde I site.  Nde I encodes HisMet so
one would provide one's coding sequences with Nde I at the initiator
Met.  This seems too good not to have been done already!

	Thanks in advance.
Charlie Brenner

Rosenstiel Center, rm 610	phone (617) 736-4944
Brandeis University		fax   (617) 736-2405
Waltham, MA 02254-9110		brenner at

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