request for selectable reporter gene

Owen Rowland owen.rowland at utoronto.ca
Thu Jun 15 17:19:22 EST 1995


To whoever reads this posting:  

I am seeking a selectable reporter gene such as HIS3 or URA3, fused to a
minimal promoter (ie. No UAS) that contains a unique cloning site upstream
of a TATA box and start sites of transcription.  I currently have a minimal
GAL1 promoter fused to HIS3, but find that this UAS-less construct is
active enough make yeast containing this plasmid HIS+. 

Is anyone using such a reporter gene for the one-hybrid system or something
similar?

Thanks for the info in advance,
Shelley Hepworth/Jackie Segall/U of T

  





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