2 micron/TRP1

Ernie Hannig hannig at UTDALLAS.EDU
Tue Jun 20 08:25:23 EST 1995


Dear yeast netters:

	Thought I'd post one more time before we make it. Does anyone 
have a 2 micron-based plasmid with a TRP1 marker. The catch is the pRS 
copy number in E. coli is too high, and leads to toxicity in E. coli once 
our gene is cloned in. "Standard" pBR origins (such as those in YEp13 
and YEp24) seem to be OK.  Thanks for checking!

........ernie


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Dr. Ernie Hannig
The University of Texas at Dallas
Molecular and Cell Biology, FO3.1
P.O. Box 830688
Richardson, TX 75083-0688
(214) 883-2505 (office)
(214) 883-2409 (FAX)
email hannig at utdallas.edu




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