PCR Replacement/Knockout

Mike Dalrymple dalrymple at pplros.demon.co.uk
Thu Jun 22 07:17:03 EST 1995


Hello,

we have dabbled in the PCR knockout/replacement approach to yeast genome
alteration
without much success. Postings on this topic appeared some months ago and
were
helpful, but I've been told that current methodologies work EXCEPTIONALLY
well. Could anyone update me on the particular parameters which lead to
success
with this technique. I understand that a paper on the technique has been
published,
is anyone familiar with it?

Many thanks

Mike Dalrymple
PPL Therapeutics Ltd
Edinburgh



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