2 micron/TRP1

S. L. Rennie rennie at bldghsc.lan1.umanitoba.ca
Fri Jun 23 17:34:34 EST 1995


In article <Pine.SUN.3.91.950620082059.14900A-100000 at sioux.utdallas.edu>,
hannig at UTDALLAS.EDU (Ernie Hannig) wrote:

> Dear yeast netters:
> 
>         Thought I'd post one more time before we make it. Does anyone 
> have a 2 micron-based plasmid with a TRP1 marker. The catch is the pRS 
> copy number in E. coli is too high, and leads to toxicity in E. coli once 
> our gene is cloned in. "Standard" pBR origins (such as those in YEp13 
> and YEp24) seem to be OK.  Thanks for checking!
> 
> ........ernie
-------------------------------

Check the vectors in Gietz and Sugino  GENE:74 1988 pg 515 if one of these
is what you are looking for please contact me.

Sharon



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