PROBLEMS WITH NOCODAZOLE
esteylis at ACPUB.DUKE.EDU
Sun Jun 25 20:59:07 EST 1995
I am having problems trying to arrest G1 synchronized cerevisiae
with nocodazole. I'm using the drug at 20 ug/ml (dissolved in a minimal
amount of DMSO) but I'm not really sure that it is staying in solution.
Do I need to use buffered media (I am currently using YNBD with the
appropriate amino acid supplements)? Is there another microtubule-specific
agent that would be easier to use?
Any responses posted to the newsgroup would be much appreciated!
Duke Univ. Medical Center/Glaxo-Wellcome, Inc.
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