G418 in S. pombe

Michael Moser moser at U.WASHINGTON.EDU
Tue Jun 27 20:29:26 EST 1995


Dear fizzies,
	I routinely use 100 mcg/ml G418 (actual G418, not G418 sulfate) in
YEA plates to select for plasmids containing neoR driven by the adh1
promoter.  I autoclave the media 1st, cool to 55 C then I add drug from a
10 mg/ml stock.  It seems to work fine although the plates do get bad
after extended storage > 1yr. 
Sincerely,

Mike Moser                                             Tel: 206-543-5354
UW Department of Biochemistry                          FAX: 206-685-1792
Box 357350                                        moser at u.washington.edu
Seattle, WA  98195-7350                     Make peace my beast is yeast




More information about the Yeast mailing list